Journal: Biosensors

Article Title: Review of Transducer Principles for Label-Free Biomolecular Interaction Analysis

doi: 10.3390/bios1030070

Figure Lengend Snippet: Overview of the commercially transducer systems for Biomolecular interaction analysis (BIA). Information is taken from the website of the companies where not stated differently.

Article Snippet: , Corning , Epic , 0.5 , 384-well microplate , n/a , 15–30 μL typical , www.corning.com , .

Techniques:

Journal: Molecular Metabolism

Article Title: High-throughput screening identifies small molecule inhibitors of thioesterase superfamily member 1: Implications for the management of non-alcoholic fatty liver disease

doi: 10.1016/j.molmet.2023.101832

Figure Lengend Snippet: Figure 1: Small molecule inhibitors targeting Them1 activity identified by high-throughput screening. Reactions were performed in 384-well microplates with recombinant His-tagged human Them1 (Them1; 125 nM), myristoyl (C14)-CoA (C14-CoA; 25 mM) and compounds (12.5 mM), and incubated for 60 min at 22 C. (A) Z0 factors were calculated from reactions containing no enzyme [(þ) control] or no C14-CoA [() control; 25 mM]. Plate ID indicates the unique number assigned for each plate in the compound library. (B) Scatter plot for the normalized percent inhibition (NPI) of 360,705 library compounds (yellow dots [(þ) control)]; green dots [() control] and gray dots [compounds (12.5 mM)]). (C) Representative 384-well microplate from the high-throughput screen. Compounds were considered as potential inhibitors based on a NPI 30%. Negative values are attributable to the absorbance of a minority of compounds. The Z0 factor is shown. (D) Representative compounds that exhibited inhibition of Them1 activity (open circles) or exceeded the maximum IC50 screening criteria of 20 mM (closed circles). Data represent mean (s.e.m.) of triplicate determinations. Where not visible, standard error bars are contained within the symbol sizes.

Article Snippet: Critical aggregation concentrations of compounds Critical aggregation concentrations of compounds were determined using dynamic light scattering in black 96-well flat bottom polystyrene microplates (GreinerBio One International AG; Kremsmünster, Austria) at 22 C using a DynaPro plate reader (Wyatt Technology; Santa Barbara, CA).

Techniques: Activity Assay, High Throughput Screening Assay, Recombinant, Incubation, Control, Drug discovery, Inhibition

Journal: Molecular Metabolism

Article Title: High-throughput screening identifies small molecule inhibitors of thioesterase superfamily member 1: Implications for the management of non-alcoholic fatty liver disease

doi: 10.1016/j.molmet.2023.101832

Figure Lengend Snippet: Figure 6: START domain-dependent inhibition of Them1 activity by freshly synthesized compound U1 and novel structural derivatives. Scaffold modifications of freshly synthesized compound U1 and novel structural derivatives (U6 e U17). Reactions were performed in 384-well microplates with recombinant His-tagged human Them1 (Them1; 125 nM), myristoyl-CoA (C14-CoA; 25 mM) and compounds, and incubated for 60 min at 22 C. Data represent the mean (s.e.m.) of triplicate determinations. Where not visible, error bars are contained within the symbol sizes.

Article Snippet: Critical aggregation concentrations of compounds Critical aggregation concentrations of compounds were determined using dynamic light scattering in black 96-well flat bottom polystyrene microplates (GreinerBio One International AG; Kremsmünster, Austria) at 22 C using a DynaPro plate reader (Wyatt Technology; Santa Barbara, CA).

Techniques: Inhibition, Activity Assay, Synthesized, Recombinant, Incubation